The Jessie Gap source-bordering/climbing dune is significant because it provides an unusual record of mid-late Holocene dynamic geomorphic processes operating in the development of Central Australian landforms. The deposition of sand on the Jessie Creek flood-out is interpreted as a result of a large magnitude palaeoflood. The subsequent formation of the dune’s upper unit from this sand through wind action, reflects the specific climatic conditions prevailing at the time. The site has the potential to further our understanding of the complex interaction of aeolian and fluvial processes and highlights the role of wind and water in shaping parts of the landscape. The site’s significance to the Aboriginal community is reflected in its recognition as a registered sacred site.